应用蚕豆根尖及叶尖细胞微核技术检测TNT及车间空气的诱变性  

DETECTING THE MUTAGENICITY OF TNT AND THE POLLUT-ED AIR INTNT WORKSHOPS WITH MICRONUCLEUS ASSAY INVICIA FABA ROOT AND LEAF TIPS

作　　者：戴敏 吴琼[1] 周心一[1] 李斌[1] 韩晓峰 

机构地区：[1]西安兵器工业卫生研究所,西北工业大学,华山机械分厂

出　　处：《癌变．畸变．突变》1995年第1期58-61,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：应用蚕豆根尖及叶尖细胞微核试验检测ＴＮＴ的致突变效应及作业车间空气的诱变性，用不同剂量的ＴＮＴ处理蚕豆根尖３．５ｈ后，其细胞微核北与阴性对照组比较差别有高度显著性（Ｐ＜０．０１），并有剂量—效应关系。将蚕豆幼苗置ＴＮＴ作业车间空气中暴露６ｈ后，各测试点叶尖细胞微核率均高于清洁区对照组，经统计学处理差别有高度显著性（Ｐ＜０．０１），且叶尖细胞微核率随空气中ＴＮＴ浓度的增高而增加。研究结果表明：ＴＮＴ明显诱导蚕豆根尖及叶尖细胞微核率的增加．显示其具有生物诱变作用。该检测方法灵敏、实验周期短、结果可靠、取材方便而经济，适用于对工业遗传毒物诱变性的快速检测及车间空气诱变性的监测。The mutagenicity of TNT and the contaminated air in TNT workshops has beendetected with the micronucleus (MN) test in Vicia faba root and leaf tip cells. After Viciafaba root tips were treated with different doses of TNT for 3.5h,the frequencies of MN weresignificantly raised from that of the negative control (P<0.01),and the dose effect rela-tionship was clear. When Vicia faba seedlings were exposed to air in TNT workshops for6h,the MN frequency in leaf tips of each monitoring group was higher than that of the cleancontrol. Statistically,the differences were significant (P<0.01),and the MN frequency wasexposure dependent.It indicates that TNT is mutagenic.

关 键 词：蚕豆 微核试验 致突变性 梯恩梯 工业毒理 TNT 

分 类 号：R994.3[医药卫生—毒理学]