中国河南西峡晚白垩世恐龙蛋化石基因分离及序列分析  

DNA Ioslation and Sequence Analysis of Dinosaur DNA from Cretaceous Dinosaur Egg in Xixia Henan,ChinaLI Yi AN Chengca i

作　　者：李毅[1] 安成才[1] 朱玉贤[1] 张昀[1] 刘一飞[1] 曲林[1] 由凌涛梁晓文 李小华 瞿礼嘉[1] 周曾铨[1] 陈章良[1] 

机构地区：[1]北京大学生命科学学院

出　　处：《北京大学学报（自然科学版）》1995年第2期148-152,共5页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：北京大学和蛋白质工程及植物基因工程国家重点实验室资助

摘　　要：从河南省西峡晚白垩世一枚恐龙蛋化石内腔絮状内含物及外壳提取ＤＮＡ样品，并用一段特异引物与６碱基随机引物进行ＰＣＲ扩增、基因克隆和序列分析。结果表明用这对引物从蛋腔絮状内含物样品中扩增到一段１５０ｂｐ的ＤＮＡ片段，推测氨基酸序列与ＥＭＢＬ库中已知蛋白质氨基酸序列进行了序列同源性分析，结果表明所克隆到的这段基因与非洲爪蟾表皮钙粘着蛋白（ｃａｄ－ｈｅｒｉｎ）前体及脑细胞粘着蛋白、牛及人胎盘胞钙粘着蛋白在氨基酸水平有显著的序列同源性，分别为４０．６％，３７．５％，３４．４％和３６．４％。DNA was extracted from 70～80 million year old dinosaur egg excavat-ed from Cretaceous belt formation in Xixia Henan.China.The DNA samples from both outer shell and inner floccule contents were used as templates in polymerase chain reactions (PCR )with a specific primer and hexamers. With these two primers a l50 bp DNA fragment has been amplified , cloned and sequenced.Deduced amino acid sequence of this dinosaur DNA has been compared with known protein se-quences in EMBL Bank. The results showed that it has significant homology with Xenopus laevis epithelia precursor-Ep-cadherin(40.6%)and brain-cadherin(37.5%)、bovine placental cadherin(34.4%)and human placental cadherin(36;4%),respectively.

关 键 词：DNA 晚白垩世 基因 恐龙蛋 序列 化石蛋 

分 类 号：Q915.21[天文地球—古生物学与地层学]