金黄地鼠坐骨神经移植段诱导运动皮质细胞和脑干神经元轴突再生的研究  

THE STUDY ON THE SCIATIC NERVE GRAFT INDUCING AXONAL REGENERATION OF THE NEURONS IN MOTOR CORTEX AND BRAINSTEM IN ADULT HAMSTERS

作　　者：陈庆山[1] 郑拱秋[1] 苏国辉 

机构地区：[1]北京医科大学解剖学系,香港大学解剖学系

出　　处：《北京医科大学学报》1995年第1期19-22,共4页Journal of Peking University(Health Sciences)

摘　　要：将右侧坐骨神经植入金黄地鼠的延髓锥体交叉处，同时，又将左侧坐骨神经切成数个短段后全段植入运动皮质。８周后用辣根过氧化物酶（ＨＲＰ）轴突逆行追踪法观察ＨＲＰ标记的轴突再生中枢神经元。结果，各组的运动皮质内均未发现标记细胞，在脑干和间脑可见标记细胞，呈距移植神经越远标记细胞越少的分布趋势。提示：植入锥体细胞胞体周围的坐骨神经短段对远距胞体的轴突损伤后再生无明显促进作用，脑干神经元的轴突再生与移植神经插入及轴突损伤部位距神经元胞体之间的距离密切相关。A segment of the right sciatic nerve was transplanted into medulla pyramidal decussation and concurrently sev-eral short segments of the left sciatic nerve were inserted into the motor cortex.Eight weeks later,1μl of 20% so-lution of HRP was injected into the sciatic nerve graft placed in the medulla.The HRP Iabelled cells were found inthe brainstem and diencephalon,but none was present in the motor cortex. Most of labelled cells were concentrat-ed around the grafting site.The number of labelled cells decreased rapidiy with increasing distance from the site ofgraft insertion。Thus,the results suggest that the insertion of the short sciatic nerve grafts near the pyramidal cellbodies dO not enhance regeneration of the neurons in the motor cortex,and the ability of the damaged axons togrow into the graft partly depends on the location of the cell bodies from the site of axotomy and the sciatic nervegraft.

关 键 词：神经再生 轴突 锥体细胞 坐骨神经 移植 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]