胎儿胸腺细胞对PWM和PHA反应性比较  

COMPARISON OF THE RESPONSIVITY OF HUMAN FETAL THYMOCYTES WITH PWM AND PHA

作　　者：孙永莲[1] 马树俊 刘白[1] 宗淑贞[1] 刘国荣 吕平 龙振洲[1] 

机构地区：[1]北京医科大学免疫学系

出　　处：《北京医科大学学报》1995年第3期171-172,175,共3页Journal of Peking University(Health Sciences)

摘　　要：比较了ＰＷＭ和ＰＨＡ对胎儿胸腺细胞及Ｔｈｙ－Ｌ（比重≤１．０７４）和Ｔｈｙ－Ｈ（比重＞１．０７４）两亚群的增殖效应。研究结果表明：ＰＷＭ在较大浓度范围内（０．２～２０μｇ／ｍｌ）对胎儿胸腺细胞尤其是Ｔｈｙ－Ｌ有强烈诱导增殖作用，ＰＨＡ的诱导增殖作用呈明显剂量依赖关系，ＩＬ－２对ＰＨＡ的协同作用远较对ＰＷＭ的协同作用强；多聚甲醛处理的Ｕ细胞对ＰＷＭ和ＰＨＡ诱导的增殖均有辅助作用；Ｔｈｙ－Ｌ为胸腺细胞中对刺激敏感的亚群，而Ｔｈｙ－Ｈ对刺激不敏感。he proliferative effects of PWM and PHA on human fetal thymocytes were compared.thymocytes were dev-ided into Thy-L(Light density thymocytes)and thy-H(High density thymocytes)subsets by Ficoll-Hypaque den-sity gradient centrifugation. The results showed that:PWM in wide range of dose(0.2～20μg/ml)could signifi-cantly stimulate human fetal thymocytes especialy Thy-L to proliferate. The effect of PHA on thymocytes prolifer-ation was dose-dependent.IL-2 could enhance the proliferation of thymocytes stimulated by PHA much morestrongly than by PWM,U cells treated by paraformaldehyde could enhance the proliferative effects of both PWMand PHA on thymocytes. Thy-L was much more sensitive to stimulators than Thy-H.

关 键 词：胎儿 胸腺细胞 细胞分裂 植物血凝素 有丝分裂原 

分 类 号：R392.12[医药卫生—免疫学]