一种中国发生的真菌传小麦花叶病毒RNA－13＇末端核苷酸序列分析  

SEQUENCE ANALYSIS OF THE 3’-TERMINAL OF FUNGUS-TRANSMITTED WHEAT MOSAIC VIRUS RNA-1ISOLATED IN CHINA

作　　者：于嘉林[1] 晏立英[1] 冯继东[1] 李大伟[1] 蔡祝南[1] 刘仪[1] 

机构地区：[1]北京农业大学农业生物技术国家重点实验室

出　　处：《病毒学报》1995年第3期248-254,共7页Chinese Journal of Virology

基　　金：国家自然科学基金

摘　　要：以采自河南的真菌传小麦花叶病毒（ＦＷＭＶ－Ｃ）为材料，抽提病毒ＲＮＡ，合成互补ＤＮＡ（ｃＤＮＡ）。对杂交筛选所得ｃＤＮＡ克隆进行亚克隆及序列分析，结果表明，亚克隆ｐＧＳＩ含有一个长度为８９１个核苷酸的不完整开放阅读框架（ＯＲＦ）和长度为２５８个核苷酸的３＇末端非编码区（ＮＴＲ），并带有Ｐｏｌｙ（Ａ）尾序。此段序列与大麦黄花叶病毒（ＢａＹＭＶ）及法国报道的一种小麦梭条花叶病毒（ＷＳＳＭＶ－Ｆ）的ＲＮＡ－１３＇末端分别具有６７．６％和６９．９％的同源性。由所测序列编码区（１－８９１ｎｔ）可推导产生２９６个氢基酸，并与ＷＳＳＭＶ－Ｆ及ＢａＹＭＶ外壳蛋白氨基酸序列分别具有７５．９％和７１％的同源性。此结果表明，ＦＷＭＶ－Ｃ为另外一种不同于ＷＳＳＭＶ－Ｆ的大麦黄花叶病毒组（Ｂａｙｍｏｖｉｒｕｓ）病毒，所测基因组片段应为ＲＮＡ－１３＇末端序列，其中可能包括了病毒全长外壳蛋白编码区域。fungus-transmitted wheat mosaic virus (FWMV-C)isolated from Henan, China was used for viral RNA extraction and complementary DNA synthesis. Sequencing of cDNAclones obtained from hybridization screen showed that the subclone of pGSI contained partial length of a single open reading frame (ORF) of 891 nucleotides and a nontranalated rogion NTR) of 258 nucleotides at the 3’-end excluding the poly(A)-tail. The nucleotide sequence shares homology of 67.6%and 69.9% with barley yellow mosaic virus (Ba YMV)RNAwheat spindle streak mosaic virus RNA 1reported in Frence (WSSMV-F)with in the same length of the 3’-terminals. 296 amino acids were deduced from the coding region (1-891nt )with sequence homology of 75.9%and 71% to WSSMV-F and BaYMV capsid protein respectively. The fragment was supposed to be the 3’-terminal of FWMV-Cgenome RNA 1 and responsible for encoding at least in part of the viral coat protein. The result showed that FWMV-C is a different wheat mosaic virus belonging to baymovirus group.

关 键 词：真菌传小麦病毒 小麦花叶病毒 RNA 核苷酸 

分 类 号：S435.121.5[农业科学—农业昆虫与害虫防治] S432.41[农业科学—植物保护]