水稻矮缩病毒基因组第九号片段的cDNA克隆及序列分析  

cDNA CLONING AND SEQUENCE ANALYSIS OF RICE DWARF VIRUS GENOME SEGMENT S9

作　　者：曲林[1] 辛毅[1] 朱玉贤[1] 陈章良[1] 

机构地区：[1]北京大学生命科学学院,蛋白质工程及植物基因工程国家重点实验室

出　　处：《病毒学报》1995年第3期271-275,共5页Chinese Journal of Virology

基　　金：国家科委863水稻抗病毒基因工程资助课题(863-101)

摘　　要：水稻矮缩病毒基因组第九号片段的ｃＤＮＡ克隆及序列分析曲林，辛毅，朱玉贤，陈章良（北京大学生命科学学院，蛋白质工程及植物基因工程国家重点实验室北京１ｌｌ８７１）关键词水稻矮缩病毒，第九号片段，序列分析水稻矮缩病毒（ＲｉｃｅＤｗａｒｆＶｉｒｕｓ，ＲＤＶ）...he cDNA of Rice Dwarf Virus (RDV)Fujian isolate genome segment S9was synthesized and amplified by RT-PCR. The PCR product was cloned and sequenced. The cloned fragment is 1,225bp in length, encodes a 39kD protein. This fragment has 93.9% homology in nucleotide sequence and 91.7% in amino acid sequence with RDV Japanese isolate S9. amino acid sequence comparison also showed that the N-terminal region of 39kD protein is highly conserved. It may suggest that the N-terminal region plays an important role in vivo in virus replication and encapsidation. Amino acid sequence comparison of proteins encoded by RDV S9 and WTV S11 also showed 32.9% homology.

关 键 词：水稻矮缩病毒 第九号片段 序列分析 CDNA 克隆 

分 类 号：S435.1149[农业科学—农业昆虫与害虫防治] S432.41[农业科学—植物保护]