1989－1994年中国脊髓灰质炎病毒的分子流行病学  

MOLECULAR EPIDEMIOLOGY OF POLIOVIRUS IN CHINAIN THE EPIDEMIC PERIOD OF 1989-1994

作　　者：郑渡平 张礼璧[2] 侯晓辉[2] 朱宏[2] Mac Otten David R Kilpatnck Mark Pallansch Olen M Kew 

机构地区：[1]江西省卫生防疫站 [2]中国预防医学科学院病毒学研究所国家脊灰实验室 [3]世界卫生组织(WHO)驻中国消灭脊灰官员 [4]美国疾病控制中心

出　　处：《病毒学报》1995年第4期289-297,共9页Chinese Journal of Virology

基　　金：国家自然科学基金

摘　　要：１９８９～１９９４年中国发生的脊髓灰质炎流行，疫情开始集中于华东、中南地区，后期趋于南部沿海一带。对近百份来自１８个省、市、自治区脊灰病毒流行期间标本的分子生物学特征分析，发现流行主要由脊灰病毒Ⅰ型野毒株引起，未发现Ⅱ型野毒株，但１９９３年在新疆分离到１株Ⅲ型野毒株。Ⅰ型野毒株中共存在４个基因型病毒，其中ＣＨＮＰ１－Ｒ９１型病毒为这几年流行中新产生的重组病毒，在ＶＰ１／２Ａ区含有一段疫苗株序列，另一段野毒株序列很可能来自ＣＨＮＰ１－ＪＸ８９型病毒。这两个基因型病毒分布在全国大部份地区。病毒核酸的亲缘关系研究还证明，９２～９４年流行在南方沿海一带的脊髓灰质炎，主要由９１年产生的重组病毒引起。此研究揭示了各地区、各基因型病毒与疫情之间的流行病学关系及传播模式。oliomyelitis was epidemic during 1989- 1994 in China's Mainland. Heavy outbreaks werelocated in the east, middle and south of China. Nearly 100 isolates from 18 provinces duringthis period were analyzed for their molecular characteristics. It was found that wild polio typeI was the major agent of polio epidemic in China. Although there was no wild type 2poliovirus found, type 3 wild poliovirus was isolated in Xingjiang province in 1993, inaddition to type 1 wild poliovirus circulating there for years. An type 1 wild poliovirusesclustered into 4 genotypes by sequence analysis: CHNP1-JX89, CHNP1-R91,CHNP1-GX90 and CHNP1-XJ90. The CHNP1 -R91 genotype included all recombinantviruses whose ancestor arose in the north of China in 1991. Further analysis by nucleic acidsequencing proved that Sabin 1 vaccine virus was involved in this eveat in which a vaccinevirus recombinted with one of wild polioviruses from the CHNP1-JX89 genotype group. Thewild polioviruses in CHNP1 -JX89 and CHNP1 -R91 were the main genotypes causing polioepidemics in China in the .period of 1989- 1994. This study first established the transmissionpattem of wild poliovirus and the molecular epidemiological relationship of polio viruses indefferent regions in China.

关 键 词：脊髓灰质炎病毒 分子流行病学 

分 类 号：R373.22[医药卫生—病原生物学]