汉滩病毒毒力标志的建立  

ESTABLISHMENT OF MARKERS FOR VIRULENCEOF HANTAAN VIRUS

作　　者：邱建明[1] 宋干[1] 杭长寿[1] 张全福[1] 霍子威[1] 桃树元 

机构地区：[1]中国预防医学科学院病毒学研究所

出　　处：《病毒学报》1995年第4期298-304,共7页Chinese Journal of Virology

摘　　要：比较了汉滩病毒Ａ９株强毒克隆病毒与弱毒克隆病毒在体外培养、诱导中和抗体能力，以及单克隆抗体（单抗）反应谱等方面的差异。发现最为明显的区别在于弱毒克隆病毒对小鼠腹腔巨噬细胞及人外周血单核细胞敏感性降低，在Ｖｅｒｏ－Ｅ６细胞上繁殖缓慢，空斑形成需较长时间，且斑小。但强、弱毒克隆病毒的致细胞融合活性，刺激ＣＴＬｓ应答活性，以及时Ｖｅｒｏ－Ｅ６细胞的吸附速率与穿透力均无明显差别。经温度敏感（ｔｓ）试验及缺损干扰（ＤＩ）颗粒检测，表明弱毒克隆病毒为非ｔｓ株，不含ＤＩ颗粒。而弱毒克隆病毒免疫６～８周龄ＢＡＬＢ／ｃ小鼠，同样能诱导较高滴度的中和抗体。强、弱毒克隆病毒的单抗反应谱表明，除了对４株抗糖蛋白单抗的反应性有显著差异外，两克隆病毒对１８株抗核蛋白单抗及１８株抗糖蛋白单抗的反应性无显著差异。ell culture prolifeation, ability for inducing neutralizing antibodies and McAb raictionprofile were compatal between the attenuated clone A39s and the virulent clone A9v ofHantaan virus isolate A9. Differences were found : the attenuated virus clone A39s lost itsinfectivity to peritoneal macrophages of BAlB/c mice and human peripheral bloodmononuclear leukocytes;A39s virus grew slowly on Vero-E6 cells, a longer time was neededto form plaques; and size of the plaques formed was much smaller than those of A9v virus.No significant alternations were observed in cell fusion function, stimulating cytotoxic cellactivity, the adsorption and penetration rate on Vero-E6 cell culture between A39s virusand A9v virus. It was demonstrated that A39s virus was not temperature-sensitive(ts)and contained no defective interfering particles(DIP). No substantial differences in antibodyresponse to these two viruses were observed, A39s virus could induce high titered neutralizingantibody and could cross-neutralize some other strains of Hantaan virus. Furthermore,theMcAb reaction profiles by indirect IFA and PRNT between these two viruses were compared.It was found that both had the same reactivity with 18 anti-nucleoprotein(NP)McAbsand 18 anti-glycoprotein(G)McAbs except 4 anti-G McAbs, 3D5, 6D4, 16DZ and 8F8which suggested that some genetic variations might have occurred on the glycoprotein of A39svirus, especially on the Gl glycoprotein.

关 键 词：汉滩病毒 毒力标志 人体病毒 

分 类 号：R373.32[医药卫生—病原生物学]