快速ELISA的建立和应用  

APPLICATION OF AN IMPROVED RAPID SHAKINGINCUBATION SYSTEM OF ELISA

作　　者：皮国华[1] 邓瑶[1] 刘加[1] 王慧娟[1] 张素香[1] 

机构地区：[1]中国预防医学科学院病毒学研究所

出　　处：《病毒学报》1995年第4期381-383,共3页Chinese Journal of Virology

摘　　要：快速ＥＬＩＳＡ的建立和应用皮国华，邓瑶，刘加，王慧娟，张素香（中国预防医学科学院病毒学研究所，北京１０００５２）关键词快速ＥＬＩＳＡ，抗－重组甲肝病毒单克隆抗体，抗－ＨＢｓ单克隆抗体１９７２年Ｅｎｇｖａｌｌ等人发展了酶联免疫吸附试验（Ｅｎｚｙｍｅ－ｌ...n efficient rapid shaking incubation system was improved for the detection of HBsAgand hybridoma cell lines producng monoclonal antibodies against recombinant hepatitis Avirus.Each step of the reaction can be completed in 30 minutes at 37℃.This shakingincubation system is a sensitive and rapid test which has been applied to produce kits for diagnosis of clinical diseases.The optimized shaking system permits economical use of reagentssince the coating antibody can be used at higher dilution than in static incubator.

关 键 词：ELISA 免疫学检验 应用 

分 类 号：R446.61[医药卫生—诊断学]