用快速低温冷冻和ESK方法分析兔脂肪栓塞脑组织中自由基  

THE ANALYSIS OF FREE RADICALS IN RABBIT BRAIN TISSUES DUE TO VENOUS FAT-EMBOLISM BY A RAPIDLY-FROZEN AND ESR TECHNIQUE

作　　者：徐杰[1] 刘桂珍[1] 詹瑞云[1] 王江 

机构地区：[1]中国科学院长春应用化学研究所应用谱学开放实验室,第一汽车集团公司医院

出　　处：《波谱学杂志》1995年第6期635-639,共5页Chinese Journal of Magnetic Resonance

摘　　要：用快速低温冷冻－ＥＳＲ实验方法研究兔脂肪栓塞模型脑组织自由基的变化，实验发现脂肪栓塞实验组自由基相对浓度是正常对照组的２－３倍。ＥＳＲ检测到脂类过氧化自由基和以碳为中心的烷基类自由基的事实表明脂肪栓塞病变存在活性氧自由基作用过程。文中对快速冷冻法可行性进行评估。A Rapidly－frozen technique and ESR method were used to directly measure free radicals generation in rabbit brain tissues due to venous fat－embolism. It had beenfound that ESR signal intensities of free radicals for the experimental fat－embolismgroup are 2－3 times higher than those for norinal contrast groups． The appearanceof the lipid peroxyl radicals and the carbon－centered alkyl radicals provides an evi－dence that thc fat－embolism pathologenesis may involve an active－oxygen radical ac－tion. In this paper the advantage of the rapidly－frozen method and its feasibility areelucidated.

关 键 词：兔 脑组织 脂肪栓塞 自由基 电子自旋共振 

分 类 号：Q954.52[生物学—动物学] O482.4[理学—固体物理]