大赖草的组织培养及植株再生的研究  

TISSUE CULTURE AND PLANT REGENERATION OF ELYMUS GIGANTEUS

作　　者：王丽[1] 邹明谦[1] 李沿宁[1] 张根发[1] 

机构地区：[1]东北师范大学,遗传与细胞研究所

出　　处：《草业科学》1995年第6期42-44,共3页Pratacultural Science

摘　　要：大赖草的成熟胚在含有２ｍｇ／Ｌ２，４－Ｄ的ＭＳ培养基上形成颗粒状愈伤组织。减少培养基中的２，４－Ｄ浓度，愈伤组织分化出绿色的芽点。生根培养基为不含任何激素的ＭＳ培养基。有分化能力的愈伤组织可以在保持培养基（ＭＳ＋２ｍｇ／Ｌ２，４－Ｄ＋０．５ｍｇ／ＬＢＡＰ）上长期保存。利用这一培养程序可以快速，大量地繁殖大赖草。文章还对外源激素在植物组织培养中的作用进行了讨论。Mature embryos of Elymus giganteus formed nodulous calli on the MS medium containing2mg/L2,4-D. The calli started to differentiate green sports of buds after reducing the level of2,4-D in the medium. The medium for inducing roots of plantlets is the free MS medium. Thecalli which have the potentiality of differentiation can be maintained in the medium with 2 mg/L2,4-D and 0.5mg/L BAP for a long period. This cultured system can be used in the fast re-production in vitro of E. giganteus.The role which exagenous hormones play in plant tissue cul-ture was also discussed in this paper.

关 键 词：大赖草 组织培养 繁殖 植株再生 

分 类 号：S543.903.5[农业科学—作物学]