识别和计数微生物细胞的伏安型生物传感器的研究  

A Study on the Voltammetric Biosensor for Detection and Classification of Microbial Cell

作　　者：谢平会[1] 许春向[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,哈尔滨150001

出　　处：《传感技术学报》1995年第3期24-29,共6页Chinese Journal of Sensors and Actuators

摘　　要：报道了一种半微分循环伏安型生物传感器，可同时完成对细胞的识别数。该传感器由一个平面热解石墨电极、铂电极及Ａｇ／ＡｇＣｌ电极的三电极系统组成。将阻留微生物细胞的滤膜紧附在工作电极表面，然后在工作电极与对极间施加一扫描电压，进行半微分循环伏安扫描，记录伏安图谱 。Detection and classificationn of microbial cell are important in clinical, envi-ronmental and bioindustrial fields. An electrochemical method for detection and classifi-cation of microbial cewlls has been developed by applying semi-differential cyclic voltam-metry to microbial cells. A modified basal plane pyrolytic graphite e1ectrode was em-ployed as a working electrode. Apply a sweep voltage between a working electrode and acounter electrode while the microbial cells on a membrane filter are brought into contactwith the working electrode. Then measure the generated current between the electrodesin phosphate buffered soline. The indexes of discrimination of microbial cells includesthe Ep(the anodic peak potential), the ep(the semidifferential anodic peak current). andthe cathodic peak in inverse voltage-scan.The anodic peak potentials of S. cerevisiae, B. subtilis and E. coli are 0. 75 V,0. 69 V, 0. 85 V(vs Ag/AgCl electrode) respectively. Therefore, these bacteria can beclearly distinguished from each other. The realationship between the peak current andthe cellconcentration of S. cerevisiae was studied. The anodic peak current was propor-tional to the cell numbers on the membrane filter in a range of (0. 5~2. 5) × 107 cells.Regression equation Y=0. 66+0. 56N(×l07), correlation coeffient 0.9901. The mini-mum detectable cell number was 2. 8 × 106 cells. The effects of modifiers was also de-scribed in this paper.

关 键 词：生物传感器 微生物细胞 识别 计数 

分 类 号：Q811.4[生物学—生物工程]