十省（区）大豆病毒毒源分析  

IDENTIFICATION OF SOYBEAN VIRUSES FROM TEN PROVINCES OF CHINA

作　　者：周益军[1] 侯庆树[1] 杨代凤 

机构地区：[1]江苏省农科院植保所

出　　处：《大豆科学》1995年第3期246-250,共5页Soybean Science

基　　金："七.五"国家攻关课题

摘　　要：采自５省（区）大豆病毒病样本６１份，经汁液接种鉴别寄主鉴定以及免疫双扩散测定，大豆花叶病毒（ＳＭＶ）单独侵染的样本３９份，黄瓜花叶病毒（ＣＭＶ）单独侵染的样本１２份，ＳＭＶ和ＣＭＶ复合侵染的有７份。从１０省（区）获得的大豆病毒病种子传毒率为２．７０％－１４．６８％，生物测定和血清学测定（免疫双扩散或ＥＬＩＳＡ）的种传样本４４个均为ＳＭＶ。选择各地有代表性的ＳＭＶ典型症状分离物，在电镜下观察粒子长度均为７５０ｕｍ左右；免疫电镜测定，ＳＭＶ抗血清可捕捉和修饰这些代表性分离物粒子。In 105 samples of soybean virus diseases (61 from infected plants of soybean and 44from seed-borne diseased seedling) from 10 provinces of China,83 were identified as soybean mosaic virus(SMV), 12 as cucumber mosaic virus(CMV)and 7 as double infectionwith SMV and CMV,by biologicyl and serological tests.All seed-transmissible sampleswere identified ad SMV.The typical SMV isolates from each province were filamentous particles about 750 um in length,which were trapped and decorated by SMV antiserum.

关 键 词：大豆 花叶病毒 黄瓜花叶病毒 种子传毒 病毒病 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]