榄香烯合并加温对H_（22）－F_（25）／L肝癌细胞体外抑癌的实验研究  

In vitro study on inhibitary effect of elemene combined with hyperthermia in H_(22)-F_(25)/L hepatomas

作　　者：梁品 吴功侃 赵荣宇 凌茂英 张成宏 

出　　处：《大连医科大学学报》1995年第1期16-19,共4页Journal of Dalian Medical University

摘　　要：利用我国中药温莪术提取的抗癌有效部位榄香烯（ｅｌｅｍｅｎｅ）合并加温，对Ｈ２２－Ｆ２５／Ｌ小鼠肝癌细胞进行体外抑癌实验。结果显示：Ｅｌｅｍｅｎｅ（５０ｕｇ／ｍｌ）或加温（≥３９０℃）均对肝癌细胞有抑癌作用，并且随着加温温度的上升或时间的延长抑癌作用愈益明显。其中以Ｅｌｅｍｅｎｅ（５０ｕｇ／ｍｌ）合并加温（４３０℃）持续１小时抑癌效果显著。其３Ｈ－ＴｄＲ癌细胞掺入率仅为０．２４％，核分裂明显抑制（Ｐ＜０．０１），作用后的癌细胞不能再传代。这结果说明Ｅｌｅｍｅｎｅ合并加温对Ｈ２２－Ｆ２５／Ｌ小鼠肝癌细胞在体外有显著的抑癌作用，说明该药具有临床热化疗前景。Elemene,extracted from the chinese medical herb curcuma wenyujin and hyperthermia was used for antitumor action on H22-F25/L hepatomas.The results showed that in vitro elemene(50μg/ml) or hyperthermia(≥39℃) had inhibitory cancer effects.But the strongest effect expressed 3H-TDR uptake rate only 0.42%,obvious division inhibitory rate(P<0.01) and losing reproductive capacity when elemene(50μg/ml) combined with heat(43℃) simultaneously for 60 minutes.The above mentioned experimental results suggested that elemene combined with heat(acted synergistieally) had the most significances of antitumor action on H22-F25/L hepatomas.In addition,elemene has no severe side effects that other anticancer drugs have.The report provides the basis for further clinical application of intraperitoneal hyperthermia with elemene in abdominal cancers.

关 键 词：榄香烯 热化疗 肝癌细胞 实验 抗癌作用 

分 类 号：R979.1[医药卫生—药品] R965.2[医药卫生—药学]