抗肾综合征出血热病毒单克隆抗体纯化制剂的免疫学活性鉴定  

Determination of Immunological Activities of Purified Monodonal Antibodies against HFRS Virus

作　　者：王海涛[1] 汪力亚[1] 吴兴安[1] 徐志凯[1] 薛小平[1] 董之昌[1] 朱俊铭[1] 陈晓玲[1] 雷继军[1] 汪美先[1] 

机构地区：[1]第四军医大学微生物学教研室,卫生部武汉生物制品研究所

出　　处：《单克隆抗体通讯》1995年第1期13-15,共3页

摘　　要：采用多种方法对两株经辛酸－硫酸按沉淀法纯化的抗ＨＦＥＳ病毒ＭｃＡｂ进行了免疫学鉴定．结果表明，纯化后的ＭｃＡｂ的免疫学活性（抗体效价）无明显改变，特别是仍保留了很高的中和活性、血凝抑制活性及对感染乳鼠的保护活性．纯化ＭｃＡｂ制剂置４℃保存。Two McAbs against HFRS virus were purified by caprylic acid and amrnonium sulfate precipitahon.Immunological charactrhations of the purified McAbs were detendned with various testS.Itwas showed that neutralhation, hemagglutinahon inhibition and protective activities(anhbody titers)of the purified McAbs were identical with the unpurified McAbs.The antibody titers were not reducedwhen the puritied McAbs were stored at 4t within one year.

关 键 词：肾综合征出血热 单克隆抗体 纯化 抗体效价 

分 类 号：R392－33[医药卫生—免疫学] R512.805[医药卫生—基础医学]