抗D型葡萄球菌肠毒素单克隆抗体的研制及其初步应用  

Development and Preliminary Application of McAb Against Staphylococcal Enterotoxin D(SED)

作　　者：董邦权[1] 李恩善[1] 韦强[1] 杨琨[1] 刘崟[1] 白钢[1] 金伯泉[1] 雷祚荣[1] 郑玉玲[1] 

机构地区：[1]西安第四军医大学免疫学教研室,北京军事医学科学院微生物流行病研究所,陕西中医药研究院生化室

出　　处：《单克隆抗体通讯》1995年第3期22-25,共4页

摘　　要：应用小鼠杂交瘤技术获得５株分泌抗Ｄ型葡萄球菌肠毒素（ＳＥＤ）ＭｃＡｂ的杂交瘤细胞（ＤＣ３、ＤＣ４、ＤＢ１１、４Ｄ３和４Ｄ５）。其中４Ｄ３为ＩｇＭ（λ），其余为ＩｇＧ１（ｋ）。这组ＭｃＡｂ除ＤＣ３外，其它４株ＭｃＡｂ识别的抗原构象表位相同，其相对亲和力依次为４Ｄ５＞ＤＣ３＞ＤＣ４＞４Ｄ３＞ＤＢｌｌ。利用抗ＳＥＤ多克隆抗体与ＨＲＰ标记的ＤＣ３和４Ｄ３（针对不同表位）混合ＭｃＡｂ建立了夹心ＥＬＩＳＡ法，并以该法检测了自临床标本中分离的８０林金葡萄菌所产生的ＳＥＤ，其产毒率为４１．３％。By hybridoma technique we have obtained 5 strains of mouse hybridoma cells(DC3,DC4,DB11,4D3 and 4D5) secreting anti-type D staphylococcal enterotoxin(SED) Mcab.The results showed:(1) There was only one strain of IgM(λ) in these McAbs,the rest were IgGl(k);(2)These McAbs could recognize identical or interrelated (in conformation) epitopes except for DC3;(3)Their relative affinities appeared as in such order:4D5> DC3>DC4>4D 3>DB 11.A double McAb Sandwich ELISA was established with polyclonal anti-SED antibody and HRP-labelled mixture McAbs of DC3 and DC4 which were respectively directed against two distinct epitopes.By this method,toxingenecity of 80 strains of staphylococcal aureus isolated from clinical specimens was tested,with a SED-producing rate of 41.3%.

关 键 词：单克隆抗体 葡萄球菌 肠毒素D 免疫诊断 

分 类 号：R392－33[医药卫生—免疫学] R378.11[医药卫生—基础医学]