单克隆抗体测定沙门氏菌鞭毛蛋白属共同抗原表位的分布特性  

Detection of the Generically-specific Common Antigenic Epitopes on Salmonella Flagellins with Monoclonal Antibodies

作　　者：焦新安[1] 王志亮[1] 杨静 文其乙[1] 蔡学忠[1] 曹军平[1] 张如宽[1] 刘秀梵[1] 

机构地区：[1]扬州大学农学院传染病学教研室

出　　处：《单克隆抗体通讯》1995年第3期4-10,共7页

基　　金：国家自然科学基金;江苏省青年科技基金

摘　　要：本研究应用抗沙门氏菌鞭毛蛋白共同抗原的４株羊克隆抗体所建立的直接ＥＬＩＳＡ法，测定了该类抗原表位在沙门氏菌属内外的分布特征。对１８４株覆盖Ａ至Ｏ６７血清群沙门氏菌、９６株其他肠道杆菌和８株革兰氏阳性菌的测定结果显示，该共同抗原表位广泛分布于沙门氏菌全属内，而在属外出现的频率很低。这类表位既存在于Ⅰ相较毛，也存在于Ⅱ相鞭毛。它们的上述分布特性不同于分型抗原Ｏ、Ｈ的分布特征。由此可见，沙门氏菌鞭毛蛋白共同抗原表位具有高度的属特异性，可作为该菌属识别标志，在沙门氏菌快速检验和抗原结构研究方面具有重要应用价值。Distribution of the generically-specific common antigenic epitopes on Salmonella flagellins was assessed by examining the binding patterns of 4 monoclonal antibodies (McAbs), designed as CB8,de7,cc6 and DD4,to a set of 184 Salmonella strains covering serogroups A to 067 and 104 nonSalmonella bacteria including Escherichia,Shigella, Citrobacter, Enterobacter,Klebsiella,Proteus,Yersinia.Serratia,Listeria,staphylococcus, and Streptococcus.It was found that All the 4 McAbs were reacting with antigenic epitopes that were common to all the Salmonella flagellins studied,yet definite negative reactions were found with non-Salmonella straills.These epitopes were clearly different from the serotypic antigens,and they presented on flagellins of both phase-Ⅰ and phase-Ⅱ.They showed the highly generic specificity of Salmonella.All the results suggested that these epitopes could be used as recognized markers for the identification of Salmonella isolates and rapid detection ofSalmonella contamination in md,feed and clinical specimens,and would be very useful in the analysis of antigenic structure of Salmonella flagellins.

关 键 词：沙门氏杆菌属 鞭毛蛋白 抗原 免疫测定 

分 类 号：R378.23[医药卫生—病原生物学]