天然麝香养香和原香的HPLC分析  

METHOD OF ANALYSTS FOR MUSK, ARTIFICIALLY BRED MUSK AND ORIGINAL MUSK BY HPLC

作　　者：吴悦[1] 吴鹏[1] 肖宣[1] 于德泉[1] 

机构地区：[1]中国医学科学院药物研究所,北京100050

出　　处：《药学学报》1989年第4期308-311,共4页Acta Pharmaceutica Sinica

摘　　要：<正> 天然麝香中麝香酮的含量是评价麝香质量的重要指标之一。我们对人工养殖麝鹿的初香(即原香,为灰白色液体)及人工养殖麝香(简称养香)中麝香酮含量进行含量测定,并与天然麝香中麝香酮含量进行比较;同时对麝香中具有药理活性的两种主要激素——△~5-雄烯-3β-The ether extract of musk, artificially bred musk and original musk reacted with 2,4-dinitrophenylhydrazine to form corresponding phenylhydrazones. The products were determined by HPLC. The conditions of quantitative analysis of muscone hydrazone and qualitative analysis of △~5-androstene-3β-hydroxy-17-one hydrazone and 3α-hydroxy-5α-androstan-17-one hydrazone, such as the choice of chromatographic columns and mobile phase were studied. The results of standard curve and recovery experiment are satisfactory. Dual detection and MS were used to identify and analyse the purity of the chromatographic peak of muscone hydrazone. Experiments of addition of standards were used to confirm the peaks of △~5-androstene-3β-hydroxy-17-one hydrazone and 3α-hydroxy-5α-androstan-17-one hydrazone.

关 键 词：天然麝香 养香 原香 色谱法 

分 类 号：R284.1[医药卫生—中药学]