毛细管GC法同时测定咪唑安定及其代谢物的血药浓度  

Determination of midazolam and its metabolites by capillary GC method in human plasma

作　　者：周静[1] 刘垣升[1] 

机构地区：[1]第二军医大学药学院药物分析学教研室,第二军医大学教学保障处

出　　处：《第二军医大学学报》1995年第2期169-172,共4页Academic Journal of Second Military Medical University

摘　　要：用气相色谱法测定了人体血浆中咪唑安定及其代谢物的含量。以Ｒｏ２１－２２１２作内标，在弱碱性条件下用氯代丁烷－二氯甲烷（７∶３）提取样品，以氮气为载气，用ＨＰ－５大口径毛细管柱和氮磷检测器进行分析。结果咪唑安定、４－羟基和α－羟基咪唑安定平均回收率分别为９９．６２％、９９．５５％和９８．８６％，最低检测限分别为１、５和５ｎｇ／ｍｌ，日内和日间变异系数均小于８％。提示该法灵敏度高，专一性强，且内源性物质和同时使用的药物均不干扰。用本法还测定了全麻择期手术患者的药代动力学参数。A GC method is deseribed for measuring midazolam and its metabolites,α-hydroxy and 4-hydroxy midazolam simultaneously in human plasma.Compounds were extracted with chlorobutane-dichloromethane (7:3) at basic PH with Ro 21-2212 added as internal standard. A portion （2 μl) was analysed with N2 as carrier gas on a wide-bore capillary column of HP-5 and N-P detector. Mean recovery was 99. 62% for midazolam, 99. 55% for 4-hydroxy midazolam, 98. 86% for α-hydroxy midazolam and the detector limit was 1, 5. 5 ng/ml, respectively. The coefficients of variation for the inter- and intra-day were all less than 8%.No interference was found from endogenous compounds, or other commonly used drugs.Those suggest that this method can be applied to therapeutic drug monitoring and pharmacokinetic studies.

关 键 词：咪唑安定 气相色谱法 药物动力学 血浆 

分 类 号：R971.2[医药卫生—药品]