水飞蓟宾及槲皮素对体外白蛋白非酶糖化的抑制作用  

The inhibitory effects of some Chinese herbs or its components on albumin nonenzymatic glycosylation

作　　者：葛勇[1] 张家庆[1] 周云平[1] 

机构地区：[1]第二军医大学长海医院内分泌科

出　　处：《第二军医大学学报》1995年第4期333-336,共4页Academic Journal of Second Military Medical University

摘　　要：利用体外白蛋白非酶糖化系统，观察到体外白蛋白非酶糖化程度与温度、葡萄糖浓度、接触时间有明确关系。发现３７℃、葡萄糖２００ｍｍｏｌ／Ｌ、４周为一周期的体外非酶糖化系统可以造成白蛋白的明显糖化，此系统可以作为体外白蛋白非酶糖化实验和药物筛选的理想体外非酶糖化系统。同时对一些中药或其成分进行的体外实验研究还表明：水飞蓟宾、槲皮素对体外白蛋白的非酶糖化有一定的抑制作用，ＩＣ_（５０）值：对棕色反应产物分别为４．２７×１０^（－６）、２．３６×１０^（－６）；对非酶糖化终产物（ＡＧＥ）荧光各为７．３０×１０^（－６）、５．４４×１０^（－５）ｍｏｌ／Ｌ。In an albumin glycating system in vitro,we found that the amount of albumin AGE has a definite relationship with glucose concentration, temperature and time. In 37℃ glucose concentration 200 mmol/L system, 4 weeks are enough to make a significant AGE elevation, but in 37℃ glucose concentration 100 mmol/L system, 12 weeks are needed. So we prefer 37℃ glucose concentration 200 mmol/L system to screen drugs. In this study, quercetin and silybin exhibited some inhibitory activities on albumin nonenzymatic glycosylation in vitro. Their IC_(50) values for AGE were 5.44×10￣(-5) and 7.30×10￣(-6) mol/L,for brown products were 2.36×10￣(-6) and 4.27×10￣(-6) mol/L, respectively.

关 键 词：水飞蓟 槲皮素 非酶糖化 中草药 

分 类 号：R285.5[医药卫生—中药学]