大鼠颅骨成骨细胞体外培养形成钙化结节的光镜电镜研究  

Mineralized matrix formed in vitro by newborn rat calvaria osteoblast:a light and electromicroscopic study

作　　者：刘祖德[1] 臧鸿声[1] 赵定麟[1] 

机构地区：[1]上海第二军医大学长征医院骨科

出　　处：《第二军医大学学报》1995年第5期466-469,共4页Academic Journal of Second Military Medical University

摘　　要：自新生大鼠颅骨分离出的成骨细胞在含５０ｍｇ／Ｌ维生素Ｃ和１０ｍｍｏｌ／Ｌβ－甘油磷酸钠的ＤＭＥＭ＋１０％ＦＣＳ培养液内逐渐出现聚集重叠而形成结节，ＶｏｎＫｏｓｓａ染色证实结节内有钙盐沉积，细胞的碱性磷酸酶染色显示结节的钙化与高活性酶的活动有关。电镜示结节内有大量的钙盐沉积在胶原纤维上，一些类似骨细胞的细胞被包埋在钙化骨质内，整个由细胞、胶原纤维和钙盐组成的三维结构类似于体内膜内成骨。实验结果提示：体外培养的鼠颅骨成骨细胞能形成钙化骨质，进一步证实所分离的细胞具有成骨细胞的性质。Osteoblastic cells isolated from newborn rat calvaria were cultured in the DMEM+10% FCS medium supplemented with 50 mg/L ascorbic acid and io mmol/L Na-Beta-glycerophosphate.Clumps and multilayer of cells became evident and the cell nodules were formed.Von Kossa staining showed positive reaction in the nodules indicating presence of calcium phosphate,and alkaline phosphatase staining demonstrated that high activity of the enzyme was associated with formation of the nodules.Ultrastructure analysis of the nodules showed mineral crystallites closely associated with collagen fibrils.Osteocyte-like cells were completely surrounded by mineralized matrix.Development of the three dimension structure consisting of the cells,collagen fibrils and mineral deposits resembled that of intramembranous osteogenesis.The results indicate that newborn rat calvaria osteogenic cells elaborate in culture a mineralized matrix,which further support the osteoblastic nature of the cells.

关 键 词：颅骨 成骨细胞 钙化 体外培养 结节 

分 类 号：R683.05[医药卫生—骨科学] R329.4[医药卫生—外科学]