肠型放射病时小鼠小肠壁内一氧化氮合酶的动态分布  

The dynamic distribution of nitric oxide synthase in the small intes-tine of mice with intestinal type radiation sickness

作　　者：魏丽春 郭鹞 

出　　处：《第四军医大学学报》1995年第1期13-16,共4页Journal of the Fourth Military Medical University

摘　　要：目的：为证明一氧化氮合酶（ＮＯＳ）在肠型放射病过程中的变化，探索其病理意义．方法：作者给小鼠以￣（６０）Ｃｏγ－射线一次全身性照射２５Ｇｙ，在照射后不同时间点取其小肠（十二指肠、空肠和回肠）作铺片和切片．用ＮＡＤＰＨ硫辛酰胺脱氢酶组织化学方法显示ＮＯＳ的分布情况．结果：与对照组相比，受照后存活４８ｈ和７２ｈ的小鼠肠肌丛内ＮＯＳ阳性神经胞体和纤维的密度明显升高，阳性胞体和纤维的染色亦加深；肠绒毛表面及肠腺腺腔内ＮＯＳ分布亦有改变．结论：结果提示，受照射小鼠小肠壁内ＮＯＳ活性的增加可能是肠型放射病晚期肠腔过度扩张，血管舒张充血的重要原因之一．Objective: For a study of the dynamic change of nitric oxide synthase(NOS)and its pathological significance during radiation sickness intestinal type. Metbods :Fifteen mice were given￣(60)Co γ-ray radiation and sacrificed after different survival times. The dynamic distribution of NOS in the small intestine (duode-num,jejunum and ileum)was studied by using NADPH-diaphorase histochemistry. Results :In the animals of the 48 h and 72 h test groups,the number of NOS-positive neuronal bodies and density of NOS-positive ibers were markedly increased in unit area of the myenteric plexus and deep muscular plexus compared with the control group The neuronal bodies and fibers were stained more densely. The NOS-positive reaction products in the cavities of intestinal glands and on the mucosa was also increased.Conclusion :The results suggested that the increase of NOS activity might be one of the main causes of intestinal and vascular dilata-tion during the radiation sickness of intestinal type.

关 键 词：放射病 肠型 一氧化氮合酶 小肠 小鼠 

分 类 号：R818.710.2[医药卫生—放射医学]