不同诱导剂体内快速诱生大鼠腹水和增加McAb产量方法的研究  

Study on rapid induced rat ascites formation and increased yield of monoclonal antibody in vivo by different inducers

作　　者：徐海峰[1] 杨为松[1] 崔龙洙[1] 尚高峰[1] 

机构地区：[1]唐都医院核医学科,唐都医院传染病科

出　　处：《第四军医大学学报》1995年第2期96-98,共3页Journal of the Fourth Military Medical University

摘　　要：作者采用诱导剂和杂交瘤细胞同步生产单克隆抗体（ＭｃＡｂ）腹水法，研究比较了不同成分诱导剂生产ＭｃＡｂ腹水的效果．结果表明以液体石蜡与不完全佐剂的等量混合剂（ＬＰＩＦＡ）诱导ＭｃＡｂ腹水产生的效果优于以不完全佐剂（ＩＦＡ）或降植烷（ｐｒｉｓｔａｎｅ）为诱导剂生产腹水的效果．ＬＰＩＦＡ法是ＩＦＡ或降植烷法腹水产量的１．４～２．２倍、ＭｃＡｂ产量的１．６～２．５倍；每鼠平均诱生腹水约５７～７６ｍｌ，其中单只最高一次达１１０ｍｌ．并且应用ＬＰＩＦＡ同步转种杂交瘤细胞生产腹水法有省时、省工的优点．The effects of rapid induced rat ascites formation and increased monoclonal antibody(McAb)anti- HFRS virus production by different inducers were studied.Incomplete Freund’s adjuvant(IFA ),pristane orv/v mixture of paraffin liquid and incomplete Freund’s adjuvant (PLIFA)were used to induce ascitic fluid in Lou/c rat. The best results were obtained following injection of 2 ml PLIFA at the moment of intraperitoneal transfer of rat hybridoma cells. Ascites production was increased by as much as 1.4～2.2 times and yield of McAb by more than 2 times compared with pristane or IFA treated rats.The mean ascites production was 57 ～76 ml per rat by PLIFA,and the highest was 110 ml.Induction of PLIFA may also save time and decrease labour intensiveness.

关 键 词：单克隆抗体 腹水 诱导剂 大鼠 生产 

分 类 号：R392－33[医药卫生—免疫学]