RT－PCR，ELISA和IFA技术应用于汉坦病毒检测的比较  

Comparison of RT-PCR,ELISA and IFA for detecting Hantavirus

作　　者：孙永涛[1] 白雪帆[1] 黄长形[1] 杨为松[1] 尚高峰[1] 李光玉[1] 张文彬[1] 

机构地区：[1]唐都医院传染科

出　　处：《第四军医大学学报》1995年第4期291-293,共3页Journal of the Fourth Military Medical University

摘　　要：作者报道反转录聚合酶链式反应（ＲＴ－ＰＣＲ），双单克隆抗体（ＭｃＡｂ）夹心法酶联免疫吸附试验（ＳａｎｄｗｉｃｈＥＬＩＳＡ）和间接免疫荧光技术（ＩＦＡ）三种方法对汉坦病毒７６～１１８株细胞培养物中病毒ＲＮＡ及抗原的检测情况，并比较了三种方法的敏感性．结果表明：ＲＴ－ＰＣＲ敏感性最高，种毒后２４ｈ内即可检出病毒ＲＮＡ；其次为夹心法ＥＬＩＳＡ，种毒后４８ｈ可检出冻融细胞上清内的病毒抗原；ＩＦＡ检出感染细胞内病毒抗原的最短时间为７２ｈ．作者对设计引物与提高ＲＴ－ＰＣＲ的敏感性进行了讨论。The sensitivities of RT-PCR, Sandwich ELISA and IFA for detecting Hantavirus RNA or antigen of virus strain 76～118 from Vero-E6 cell culture were compared.The results showed that the sensitivity of RT-PCR is the highest.It can detect the virus RNA in 24 h after the Vero-E6 cells were inoculated with Hantaan virus.The next is Sandwich ELISA, which can detect the virus antigen in 48 h after inoculation. It needs more than 72 h for IFA to detect virus antigen after Vero-E6 cells were infected. The relationship be-tween oligonucleotide primer design and sensitivity of RT-PCR was discussed.

关 键 词：汉坦病毒 病毒检测 聚合酶链反应 

分 类 号：R512.803[医药卫生—内科学] R373.32[医药卫生—临床医学]