恶性疟原虫杂合45肽抗原基因重组减毒鼠伤寒沙门氏菌在家兔免疫应答的初步研究  

Immunogenicity of recombinant S. typhimurium expressing a P. falciparum hybrid 45-peptide antigen

作　　者：黄建生[1] 王昌才[1] 李全贞[1] 任大明[1] 钟雄林[1] 陈仕荣[1] 李英杰[1] 

机构地区：[1]第一军医大学分子生物学研究所,第一军医大学热带医学研究所,第一军医大学复旦大学遗传所国家重点实验室

出　　处：《第一军医大学学报》1995年第3期225-227,共3页Journal of First Military Medical University

摘　　要：已经在减毒鼠伤寒沙门氏菌ＳＬＳ３６１以融合蛋白的形式表达了人工合成的恶性疟原虫杂合抗原基因Ａ。活菌以２×１０￣９ｃｆｕ（ｃｏｎｏｌｙｆｏｒｍｕｎｉｔｓ）经口服免疫新西兰家兔，用ＥＬＩＳＡ法测定血清中抗体水平，结果于首次免疫或加强免疫后都可检测到一定水平的特异性抗体，并可诱发出针对融合蛋白和恶性疟原虫抗原的迟发性超敏反应（ＤＴＨ）。活菌苗免疫后未见明显的毒副作用。45-pepetide Plasmodium falciparum antigen(Pf Ag)was expressed as a fusion protein in attenuatedSalmonella typhimurium SL3261.Live organisms were used to orally immunize rabbits with a dose of 2 ×10￣9 cfu. Specific humoral immtinity was detected when ELISA was used to measure the titre of the anti-serum. Obvious delayed type hypersensitivity(DTH)could be induced by Pf Ag and GZ-A hybrid antigen. No evident side-effects were found after oral immunization.

关 键 词：鼠伤寒沙门氏菌 恶性疟原虫 免疫应答 活菌苗 

分 类 号：R392-33[医药卫生—免疫学] R978.61[医药卫生—基础医学]