猪体外受精精子冷冻后的超微结构研究  

AN ULTRASTRUCTURAL STUDY ON IN VITRO FERTILIZATION PORCINE SPERMATOZOA AFTER CRYOPRESEPVATION

作　　者：秦鹏春[1] 谭景和[1] 吴光明[1] 徐立滨[1] 杨庆章[1] 

机构地区：[1]东北农业大学生物工程系,华南师范大学激光生命科学实验室

出　　处：《东北农业大学学报》1995年第4期377-382,共6页Journal of Northeast Agricultural University

基　　金：农业部"七.五"生物技术重点科研资助

摘　　要：猪鲜精冷冻后，虽保持了一定的活力，但受精能力明显降低。精子结构特别是顶体遭到不同程度的损伤。应用超微结构方法，观察了鲜精体外获能顶体反应（对照组）及冻精解冻（实验组）后顶体结构的改变。二者在质膜膨胀断裂、囊泡化方面，有着十分相似之处，显示冷冻解冻后，可以使有些精子产生一种假顶体反应（ＰｓｃｕｄｏａｃｒｏｓｏｍａｌＲｅａｃｔｉｏｎ），从而导致受精率下降。The fertilizing competence of porcine speermatozoa was reduced and their structure,especially that or the acrosome, was damaged to different degree after frozenthawed.Very similiar ultrastructural changes were found in the acrosome between soon after thawing(Experimental group)and during in vitro fertilization of fresh semen(Cortrol group)and these included swelling,breaking of the plasma membrane and vesiculation of the outer acrosomal membrane.This indicated that the freezing procedure had made some spermatozoa undergo a pseudoacrososmal reaction which reduced the fertilization rate.

关 键 词：猪 精子 低温冷冻 假顶体反应 超微结构 

分 类 号：S828.34[农业科学—畜牧学] S852.16[农业科学—畜牧兽医]