福建产圆斑蝰蛇毒中性磷脂酶A_2的降压作用与机理  

THE HYPOTENSIVE EFFECT AND ITS MECHANISM OF A NEUTRAL PHOSPHOLIPASE A_2 FROM SNAKE VENOM VIPERA RUSSELI SIAMENSIS

作　　者：杨小毅[1] 杨永宗[1] 刘广芬[2] 王晴川[2] 

机构地区：[1]衡阳医学院心血管病研究所 [2]福建医学院蛇毒研究室,福州350004

出　　处：《动物学报》1995年第4期375-380,共6页ACTA ZOOLOGICA SINICA

基　　金：湖南省教委科研基金资助;基金批文号1190202

摘　　要：福建产圆斑蝰蛇毒中性磷脂酶Ａ２（ＰＬＡ２－３）对麻醉Ｗｉｓｔａｒ大鼠、猫和家兔均产生急性降压效应，并具快速耐受性。该降压作用能被吲哚美辛取消。应用放射免疫法测定血浆前列环素（其稳定产物６－ｋｅｔｏ－ＰＧＦｌａ）含量，与给药前相比，降压高峰时血浆６－ｋｅｔｏ－ＰＧＦＩｌａ含量增高。离体入胃网膜动脉条实验观察到，ＰＬＡ２－３呈剂量依赖性降低入胃网膜动脉条静息张力；对预先用去甲肾上腺素或５－羟色胺收缩的人胃网膜动脉条也具松弛作用。结果表明该ＰＬＡ２降压机制与前列环素（ＰＧＩ２）的释放和外周血管扩张有关。A neutral phospholipase A, (PLA,) was purified from snake venom Vipera russeli sia mensis. The PLA, showed hypotensive actions which were abolished by indomethacin in anaesthetized rats. cats and rabbits. The tachyphylaxis of the hypotensive responses to PLA2 was also found. The plasma contents of prostacyclin (PGI2) at the time of maximal hypotension induced by PLA, were significantly higher than those before injecting the drug. The PLA, produced dose-dependent relaxations of isolated human gastro-epiploic arterial strips of both resting tension and precontracted state treated by norepinephrine or 5- hydroxytryptamine. These results suggest that the PLA,-induced hypotension may be related to the release of PGI2 in vivo and the relaxation of peripheral blood vessels.

关 键 词：磷脂酶A2 圆斑蝰蛇 蛇毒 血压 药理学 

分 类 号：R977.3[医药卫生—药品] Q959.620.6[医药卫生—药学]