高分子聚合物HFMC注入猕猴输精管后附睾的远期组织病理学变化  

LONG-TERM HISTO-PATHOLOGICAL CHANGES OF RHESUS MONKEY EPIDIDYMIDES BY INTRA-VASA DEFERENS INJECTION OF HFMC

作　　者：岳利民[1] 何亚平[1] 罗自强[1] 姚先莹[1] 李光蓉[1] 张金虎[1] 白寿昌[1] 邹淑荃[1] 徐若璞[1] 

机构地区：[1]华西医科大学,中国科学院昆明动物研究所,中国科学院成都有机化学研究所

出　　处：《Zoological Research》1995年第2期173-177,共5页动物学研究（英文）

摘　　要：本实验采用７只成年雄性猕猴，经双侧阴囊上方切口暴露输精管，由近附睾端向远侧注射高分子聚合物ＨＦＭＣ，剂量分别为３０ｍｇ／侧（１只），６０ｍｇ／侧（３只），１００ｍｇ／侧（３只），分别于术后２．５及３．５年处死动物，取附睾组织进行光镜及电镜观察。结果表明：注射不同剂量ＨＦＭＣ２．５年后，动物附晕头、体、尾各部光镜观察所见主要改变为上皮细胞局灶性轻度水样变性，少许上皮细胞脱落，个别管腔扩张，局部间质少量炎细胞浸润。３．５年后取材光镜观察附睾组织无明显异常。不同时间取材进行电镜观察所见超微结构变化主要为上皮细胞内线粒体肿胀，部分线粒体及内织网呈空泡改变，附睾管腔内精子均有一定程度损伤。上述结果提示：ＨＦＭＣ注入输精管后，由于释放Ｈ＋及暂时阻断输精管可能使附睾组织结构受到一定程度影响，并且直接或间接地影响附睾精子赖以成熟或贮存的内环境。这可能是ＨＦＭＣ输精管注射节育的可能机理之一。随着ＨＦＭＣ的消失，组织结构可能逐渐恢复。Seven male rhesus monkeys were used for the investigation of the histopathological changes of epididymides in the 2. 5th and 3.5th years after intra-vasa deferens injection of high molecular polymer HFMC. Various dosages of HFMC (Hydrogel for male contraception) were given to the animals: 30mg per vas deferens for 1 animal, 60mg for 3 and 100mg for 3.The light microscopic observation showed that there were slight local watery degeneration of epididymal epithelium, falling of some epithelial cells and expansion of lumen in some epididymal tubules in the 2.5th year following intra vas injection of HFMC but no obvious abnomality was found under light microscope in the 3.5th year.The main ultrastructure changes in the 2.5th year showed the swelling of mitochondria, slight vacuolar change of endoplasmic reticulum and damage of sperms in the epididymal tubles. The ultrastructure changes in the 3.5th year were similar to those in the 2.5th year.It is suggested that HFMC may directly or indirectly affect the epididymal structure and the epididymal enviroment due to its gradual release of H+ as well as its partial and temporary occlusion of vasa deferens. This may be one of the reasons for its male contraceptive action. However, it seems that pathological may be restored after HFMC is progressively dissolved and excereted.

关 键 词：高分子聚合物 HFMC 猕猴 附睾 避孕药 

分 类 号：Q959.848[生物学—动物学] R979.21[医药卫生—药品]