青霉素G酶促裂解液的高效液相色谱分析  

Analysis of Enzymatic Hydrolysate of Penicillin G by Reversed-phase High Performance Liquid Chromatography(RP-HPLC)

作　　者：王秀英[1] 

机构地区：[1]天津大学化学工程研究所

出　　处：《分析测试学报》1995年第4期79-81,共3页Journal of Instrumental Analysis

摘　　要：建立了用反相高效液相色谱测定青霉素Ｇ酶促裂解产物的方法。以与分离程度和分析时间有关的ＣＲＦ为目标函数，用改进的单纯形法对流动相进行优化选择。结果表明本法快速、准确、简便。A RP-HPLC method for analyzing enzymatic hydrolysate of penicillin G was established.It involves optimization of mobile phase by modified simplex method and using CRF as the objective function based on peak separation and analysis time,The advantage of the method is simple,rapid and accurate.

关 键 词：高效液相色谱 青霉素G 氨基青霉素烷酸 

分 类 号：TQ465.1[化学工程—制药化工] O657.72[理学—分析化学]