PyGC对昆虫包涵体病毒的模式识别  

Recognization of Patterns of Insect Inclusion Body Viruses by Pyrolysis Gas Liquid Chromatography

作　　者：朱湘民[1] 汤显春[1] 刘进学[1] 夏祥明[1] 陈华[1] 张世敏[1] 

机构地区：[1]中国科学院武汉病毒研究所,湖北省公安厅刑侦科学研究所

出　　处：《分析测试学报》1995年第6期37-42,共6页Journal of Instrumental Analysis

基　　金：中国科学院技术条件局资助

摘　　要：用裂解毛细管色谱法对２９株核型多角体病毒、１６株颗粒体病毒和１１株质型多角体病毒进行了表征分析，通过分析裂解图特征峰，可区分ＮＰＶ、ＧＶ和ＣＰＶ彼此间的差异和相同亚群不同分离株间的异同，用聚类分析可进一步证明ＰｙＧＣ对昆虫包涵体病毒模式识别的可行性。为分类鉴定病毒及其生化特性提供了一种现代分析方法。The analysis of 16 strain granulosis virusis,29 strains nuclear polyhedrosis viruses and 11 strains Cytoplasmic Polyhedrosis viruses were performed on a Shimadzu GV-9A capillary gas chromatography equipped with a pyrolyser midel PYR-2A. The differences among the inclusion bodies of NPV,GV,and CPV could clearly be distinguished and also be identified through analysis of”fingerprinting”.The results suggest that it is feasible to recognize pattterns of insect clusion body viruses by pyrolysis gas liquid chromatography. This procedure has provided a usefel method for the classification and identification of viruses and their biochemical characters.

关 键 词：气相色谱法 包涵体病毒 病毒 昆虫 识别 

分 类 号：Q939.42[生物学—微生物学]