胆绿素的溶出伏安法研究及胆红素的间接测定  

Investigation of Biliverdin by Adsorptive Stripping Voltammetry and Indirect Determination of Bilirubin

作　　者：鲁贤丹 曾百肇[1] 张悟铭[1] 周性尧[1] 

机构地区：[1]武汉大学化学系,武汉430072

出　　处：《分析化学》1995年第1期39-41,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金

摘　　要：本文对胆绿素的吸附溶出分析法进行了研究.发现胆绿素在Na_2HPO_4-Na_3PO_4(0.1mol/L,pH11.5)缓冲溶液中可在-0.83V(vs.SCE)左右产生灵敏的吸附溶山峰,该峰可检测出低至5×10^(-9)mol/L胆绿素。在通常条件下,通过0.5min预富集后溶出,可使峰高提高约一个数量级.应用此法间接测定了小牛血清中的胆红素含量.A stripping voltammetric method for the determination of biliverdin at submicro-molar and nanomolar concentration level is described. The method is based on the adsorption accumulation of biliverdin at a static mercury drop electrode. A detection limit of 5×10-9 mol/L has been obtained after 5 min adsorption accumulation in sodium phosphate solution (pH 11. 5). This method has been applied to the indirect determination of the bilirubin concentration in a bovine serum sample.

关 键 词：胆绿素 胆红素 伏安法 测定 

分 类 号：Q51[生物学—生物化学] O657.1[理学—分析化学]