Nafion化学修饰圆盘预富集-石墨炉 原子吸收联用法对牛血超氧化物歧化酶中铜、锌分布状态的研究  

Study on Distribution State of Copper and Zinc in Bovine Superoxide Dismutase by Graphite Furnace Atomic Absorption Spectrometry

作　　者：徐通敏[1] 周天舒[1] 金利通[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《分析化学》1995年第2期123-127,共5页Chinese Journal of Analytical Chemistry

摘　　要：本文研究了Nafion化学修饰钨丝圆盘预富集-石墨炉原子吸收(GFAAS)方法测定牛血超氧化物歧化酶(SOD)中游离态Cu～(2+)及Zn～(2+)的方法,并用GFAAS法直接测定SOD中铜、锌的总量,证实了牛血SOD中金属辅基铜、锌原子个数比为1:1,初步探讨了一定浓度的牛血SOD中Cu～(2+)、Zn～(2+)的表观离解平衡常数。为提高SOD的活性和稳定性的研究,提供了一种有效的方法。A method for the determination of free Cu2+ and Zn2+ in bovine superoxide dismu-tase (SOD) solution by GFAAS after preconcentration on Nafion chemically modified tungsten coil is described. The total amount of Cu2+ as well as that of Zn2+ is determined directly by GFAAS. It proves that the atom ratio of Cu and Zn in bovine SOD is 1 : 1. The apparent dissociation constant of Cu2+ and Zn2+ in SOD is also discussed. It provides an effective method to improve the study of SOD' s activity and stabilization.

关 键 词：铜 锌 GFAAS 超氧化物歧化酶 牛血 离解平衡常数 

分 类 号：O629.8[理学—有机化学] O657.31[理学—化学]