小剂量单次全身照射诱导小鼠免疫适应性反应的剂量与时间研究  

CHARACTERISTIC OF IMMUNOLOGICAL ADAPTIVE RESPONSE INDUCED BY LOW LEVEL WHOLE BODY IRRADIATION wiTH X-RAYS IN MICE

作　　者：鞠桂芝[1] 宋春华[1] 齐进[1] 刘树铮[1] 

机构地区：[1]白求恩医科大学卫生部放射生物重点实验室

出　　处：《辐射研究与辐射工艺学报》1995年第2期95-98,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金

摘　　要：采用Ｘ射线单次全身照射，观察诱导小鼠免疫适应性反应的预照射剂量（Ｄ１剂量）范围，损伤性剂量（Ｄ２剂量）范围，及Ｄ１与Ｄ２最佳间隔时间。实验证实，当Ｄ２为１．５Ｇｙ时，０．０２５～０．１Ｇｙ（剂量率０．０１２５Ｇｙ／ｍｉｎ）可诱导胸腺细胞自发增殖及脾细胞对ＬＰＳ的适应性反应。当Ｄ１为０．０７５Ｇｙ时，Ｄ２在１．０～１．５Ｇｙ（剂量率０．３３Ｇｙ／ｍｉｎ）范围内，胸腺细胞自发增殖及脾细胞对ＣｏｎＡ、ＬＰＳ反应的适应性反应存在。当Ｄ１为０．０７５Ｇｙ，Ｄ２为１．５Ｇｙ时，诱导适应性反应的Ｄ１与Ｄ２最佳时间为６～１２ｈ。In the present study the range of preirradiated doses (D1) andchallenge doses (D2) for the induction of immunological adaptive response and theoptimum time intervals between D1 and D2 were investigated in Kunming mice.The results were as follows. 1. Single whole body preirradiation by X-rayswith D1 doses of 25-100 mGy (12.5 mGy/min) could induce adaptive responseof spontaneous incorporation of 3H-TdR into thymocytes and the reaction ofsplenocytes to LPS. 2. With D, doses of 1 .0 to 1.5 Gy, the adaptive response ofspontaneous incorporation of 3H-TdR into thymocytes and the reaction ofsplenocytes to ConA and LPS could be induced. 3. The optimum interval for theinduction of immunological adaptive response be tween D1 and D2 could be 6-12 h.

关 键 词：小剂量辐射 免疫适应性反应 胸腺 脾脏 

分 类 号：Q691.3[生物学—生物物理学] Q274