改良DNA诱动系统pBW_（101）和pBW_（102）质粒的构建  

Construction or Improved Mobilizable DNA Mobilization System-plasmids or pBW_(101) and pBW_(102)

作　　者：温彩霞[1] 詹惠莺 

机构地区：[1]福建医学院基因工程研究室

出　　处：《福建医学院学报》1995年第1期45-47,共3页

摘　　要：利用ｐＳＵＰ５０１１质粒上的转座子Ｔｎ５－Ｍｏｂ和ｐＴＢ５上的ＴｎＨｇ分别对Ｐ＇ｔｓＬａｃ和ＲＰ４质粒进行转座和接合传递试验，通过含药的中国蓝平板对接合子进行筛选，获得１５株Ｋｍ耐药，Ｃｍ、Ａｐ敏感，在中国蓝平板上显蓝色的菌株，质粒检测证实为Ｆ＇ｔｓＬａｃ∷Ｔｎ５－Ｍｏｂ质粒。同时获得１株转座子ＴｎＨｇ插在ＲＰ４质粒的Ｋｍ抗性基因上的ＲＰ∷ＴＨｇ质粒，该质粒可形成一个比较方便的ＤＮＡ诱动系统。In this paper,the transposon of Tn5-Mob in plasmids pSUP5011 and TnHg in PTB have been transposed and conjugated into plasmids F'tsLac and RP4 respectively. 15 strains that resistant to Kanamycin but susceptible to chloromycetin ampicillin are obtained by selecting on China blue plate.Detection of the plasmids proved that they are the plasmids of F’tsLac∷ Tn5-Mob.Meanwhile, one strain that transposon TnHg has transposed to the kanamycin resistant gene in plasmid RP4 was obtained.This pair of F'tsLac∷Tn5-Mob and RP4∷TnHg can provide a convenient mobilizable DNA mobilization system.

关 键 词：药敏试验 细菌 pSUP5011 RP4 诱动系统 DNA 质粒 

分 类 号：R915[医药卫生—微生物与生化药学]