抗人Ig轻链系列单克隆抗体的研制1．抗人λ轻链亚型／亚群单克隆抗体的制备及特性鉴定  

Studies on Monoclonal Antibodies Against Human Ig Light Chains I.Preparation and Characterization of Monoclonal Antibodies Against Subtypes/Subgroups of Human λlight Chain

作　　者：赵蓉[1] 郑志竑[1] 谢捷明[1] 包少佳 吴国华[1] 邱文萱 

机构地区：[1]福建医学院肿瘤研究室

出　　处：《福建医学院学报》1995年第2期111-113,共3页

基　　金：福建省自然科学基金

摘　　要：采用ＤＥＡＥ５２提取的多发性骨髓瘤（ＭＭ）患者尿本周蛋白为免疫原，应用鼠－鼠杂交瘤技术，建立４株能分泌抗人λ轻链单克隆抗体细胞株，经鉴定，为抗人游离λ轻链亚型／亚群抗原决定簇单克隆抗体细胞株。Using mouse-motise hybridization technique with purified λchain isolated from urinary BJP of MM patient by DE52 chromatography as immunogen.Four strains of hybridoma secreting stably McAb against human λchain were established.Primary characterization showed these antibodies were against different given subtype/subgroup of hman freeλchain.

关 键 词：免疫球蛋白 λ链 抗原决定族 单克隆抗体 制备 

分 类 号：R392-33[医药卫生—免疫学]