人胚胸腺组织培养及其移植恒河猴的实验研究  

An Experimental Study on Human Fetal Thymus Tissue Culture and It Transplantation into Fujian Rhesus Monkeys

作　　者：谢建武[1] 林隽[1] 陈碧芬[1] 黄秀兰[1] 吴翊钦[1] 龚华实[1] 

机构地区：[1]福建医学院分子医学研究室,福建医学院病理解剖学教研室,福建医学院实验动物中心,福建医学院电子显微镜室

出　　处：《福建医学院学报》1995年第4期303-308,共6页

基　　金：福建省教委科研基金

摘　　要：应用组织培养，发现人胚胸腺组织贴块法培养呈典型的３个过程。培养１周后胸腺基质细胞（ＴＳＣ）大量生长，其胞浆ｃｙｔｏｋｅｒａｔｉｎ呈弱阳性表达，电镜观察ＴＳＣ细胞胞浆含有典型的张力微丝束，ＴＳＣ细胞培养上清液能促进肿瘤浸润淋巴细胞（ＴＩＬ）的体外增殖和表型转化。胸腺块悬浮培养能使胸腺上皮成分集中，ＨＬＡ－ＤＲ阳性细胞比例下降。用１２号针头对恒河猴三角肌行移植实验，结果表明移植是安全的，且胸腺上皮组织能在肌组织中生长，但仅维持１周，２周后呈条索样萎缩。In this paper,primary culture technique,histology,immunohistochemistry and transmissional electron microscopy were employed to study the human fetal thymus cultures and its transplantation.The results were as follow:(1)human thymus tissue cultures had typically three different courses by an explant technique;(2)thymus stromal cells(TSC)migrated from thymus explant after 5 days cultured in vitro(DIV)ai1d proliferated to a confluent monolayer in 10 DIV.Ultrastructurally,there were abundant bundles of typical tonofilaments,vesicles,Golgi complex,mitochondria in the cytoplasm of TSC.Cytokeratin was slightly positive in the cytoplasm of TSC;(3)TILs were isolated from metastatic lymphnodes of 2 cases of gastric carcinoma and 3 cases of colonic carcinoma.Supernatants of TSC culture media could enhance the proliferation of TILs and promote the percentage of CD and CD cells;(4)suspension culture,can make the epithelial elements concentrate and the HLA-DR positive cells decrease;(5)the cultured thymus epithelial tissues could survive during the first week after being transplanted into the deltoid muscle of Fujian Rhesus monkeys,but were accompanied by hyperemia,edema and imflammatory cell infiltrating.And the inplants atrophied two weeks after transplantation.

关 键 词：胸腺 组织培养 组织移植 恒河猴 

分 类 号：R392－33[医药卫生—免疫学] TQ464.5[医药卫生—基础医学]