外源基因在球形芽孢杆菌中的转化  

Elimination of plasmid and transformation into Bacillus sphaericus

作　　者：张培德[1] 苑小林[1] 陈石根[1] 

机构地区：[1]复旦大学生物化学系

出　　处：《复旦学报（自然科学版）》1995年第4期417-422,共6页Journal of Fudan University：Natural Science

摘　　要：为了扩大芽孢杆菌属的受体系统范围，对球形芽孢杆菌进行了质粒消除，并用含有溶葡球菌酶基因的质粒ｐＥ１９４ｃｏｐ６－１转化。结果表明，该酶基因在球形芽孢杆菌中获得了稳定、高效表达。In order to expend the host system,plasmid in B. sphaericus was eliminated and then was transformed with plasmid pE194 cop 6-1 containing lysostaphin gene into this strain. It was showed that the lysostaphin gene could express stably in high level in Bacillus sphaericus.

关 键 词：球形芽孢杆菌 质粒消除 外源基因 蛋白酶 

分 类 号：Q939.124[生物学—微生物学]