流动注射停流荧光动力学分析法测定痕量铁的研究  

Studies on Flow-injection Stopped-flow Florescence Kinetic Method for Determination of Trace Iron

作　　者：黄厚评[1] 蔡汝秀[1] 曾云鹗[1] 高登云[1] 

机构地区：[1]武汉大学化学系

出　　处：《高等学校化学学报》1995年第1期47-49,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：利用自行组装的流动注射停流荧光动力学分析装置，研究了铁（Ⅲ）与３，５－二溴水杨基荧光酮的荧光动力学反应性质，提出了一种快速、简便、精确地测定痕量铁的新方法．其线性范围为０．０２０～０．２５０μｇ／ｍＬ，线性相关系数为０．９９９，检测限为１０ｎｇ／ｍＬ，以０．２００μｇ／ｍＬ的铁（Ⅲ）作精度试验，Ｒ．Ｓ．Ｄ％＝２．１％（ｎ＝１１）．该方法已成功地用于铝合金及桃树叶中痕量铁的测定，结果分别与ＩＣＰ法和标准参考结果相符．A new flow-injection stopped-flow fluorescence kinetic method for determination of trace iron(Ⅲ) was proposed. The method is based on monitoring the binary fluorescence complex formation of Fe(Ⅲ) with 3,5-dibro mosalicylfluorone(DBSAF) at 510 nm/510 nm in an acidic medium.The linearity range is between 0.020 and 0.250 μg/mL with a correction coefficient of 0.999 and a detection limitation of 10 ng/mL. The method has been ap plied to the determination of Fe(Ⅲ)in alluminum alloy and peach leaves, the results are in agreement with ICP method and standard reference value, respectively.

关 键 词：荧光分析 铁 二溴 水杨基荧光酮 

分 类 号：O614.811[理学—无机化学] O657.31[理学—化学]