高效毛细管电泳－电化学检测对多羟基抗生素分离测定的研究  

Determination of Polyhydroxy Antibiotics by Capillary Electrophoresis with Amperometric Detection at a Copper Electrode

作　　者：方禹之[1] 方晓明[1] 叶建农[1] 

机构地区：[1]华东师范大学化学系

出　　处：《高等学校化学学报》1995年第10期1514-1518,共5页Chemical Journal of Chinese Universities

基　　金：中国科学院长春应用化学研究所电分析化学开放实验室

摘　　要：用自制的高效毛细管电泳－电化学检测装置，以３５０μｍ铜圆盘电极为工作电极，在碱性溶液中，对７种多羟基抗生素的分离进行了研究。结果表明：浓度线性范围可达２至３个数量级，最低检测限为５．０×１０^（－７）ｍｏｌ／Ｌ。在８ｈ内连续测定，峰电流和迁移时间的相对标准偏差分别为７．６％和２．２％。实际样品（丁胺卡那霉素）测定回收率为９９．６％。Capillary electrophoresis(CE) was employed to separate polyhydroxy antibiotics in alkaline solution, combined with electrochemical detection at copper electrode. The electrode used was a 350 μm diameter Cu disk operated in a wall-jet configuration. A sample mixture containing 7 different polyhydroxy antibibeics was separated within 30 min. The linearity varied over a 2-3 order of magnitude and the detection limits were 0.5 μmol/L.The reproducibility for the peak current and the migration time of 50 μ mol/L doublemycin injected every hour in a period of 8h, was 7.6%and 2.2%RSD, respectively, and the recovery of the determination of the real sample was 99.6%.

关 键 词：毛细管电泳 电化学检测 多羟基 抗生素 

分 类 号：R978.1[医药卫生—药品] O658.9[医药卫生—药学]