环氧乙烷四氢呋喃共聚醚对雄性小鼠生殖毒作用的研究  

STUDY ON REPRODUCTIVE TOXICITY OF POLY-TETRAHYDRO FURANE-ETHYLENE OXIDE IN MALE MICE

作　　者：谭明家[1] 张招弟[1] 蒋芸[1] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所,同济医科大学工业毒理研究室

出　　处：《工业卫生与职业病》1995年第3期142-145,共4页Industrial Health and Occupational Diseases

摘　　要：实验研究了环氧乙烷四氢呋喃共聚醚（ＰＴＥ）对雄性小鼠的生殖毒作用。结果表明：６５ｍｇ／ｋｇ剂量组，ＬＤＨ总活力及ＬＤＨ—Ｘ、ＳＤＨ、γ—ＧＴ活性、ＭＤＡ含量、血清睾丸酮水平均未见明显改变。剂量增加至１３０ｍｇ／ｋｇ时，在停止染毒后２４小时，仅ＬＤＨ—Ｘ的活性增高（Ｐ＜０．０５）。剂量为２６０ｍｇ／ｋｇ组停止染毒后２４小时，ＬＤＨ—Ｘ活性增高（Ｐ＜０．０５），但ＳＤＨ活性降低（Ｐ＜０．０５），２周后均恢复至正常。γ—ＧＴ活性在停止染毒后２４小时、２周、４周均增高（Ｐ＜０．０５）。病理组织学检查仅在２６０ｍｇ／ｋｇ组光镜下可见曲细精管内细胞层次减少，电镜下可见生精细胞线粒体轻度肿胀，支持细胞溶酶体增多。提示ＰＴＥ对雄性小鼠生殖系统有轻微可逆的毒作用，酶为敏感指标，ＬＤＨ—Ｘ可作为ＰＴＥ睾丸损伤的早期指标，γ—ＧＴ为恢复期指标。The reproductive toxicity of Poly-Tetrahydrofurane-Ethylene-Oxide (PTE) in male Kunming mice was studied. The results demonstrated that there were no significant differences in serum testosterone level, malonic dialdehyde (MDA) content and lactate dehydrogenase (LDH), lactate dehydrogenase X(LDH-X), sorbitol dehydrogenase (SDH), γ-glutamyltranferase (γ-GT) activities between the PET group and the negative control group at the dosage of 65mg/kg at any time. LDH-X activity elevated significantly (130mg/kg) at 24 hours after the final exposure. The activity of LDH-X remarkbly increased but SDH decreased in the group of 260mg/kg at 24 hours after the final exposure. Subsequently, the activities of LDH-X and SDH were resumed. Whereas, at the same group, γ-GT activity was significantly increased at any time. Light and electron microscopic examination might reveal slight alteration of germ cells and Leydig cells only in the group of 260 mg/kg.From above observation, it is suggested that: PTE could induce slight reproductive toxicity in mice, especially to sperm special enzyme, LDH-X might act as the sensitive primary damage index of testis and γ-GT might act as its recovery index.

关 键 词：PTE 共聚醚 雄性 生殖系统 毒性 精子特异酶 

分 类 号：R994.3[医药卫生—毒理学]