增强型荧光显微镜的量化处理及测试  

Digital Processing and Testing of Enhanced Fluorescence Microscope

作　　者：孙江政[1] 王苏生[1] 俞信[1] 陈天明 曹恩华[1] 许娜飞[1] 李景福[1] 

机构地区：[1]北京理工大学工程光学系,中国科学院生物物理研究所

出　　处：《光电工程》1995年第5期59-64,共6页Opto-Electronic Engineering

摘　　要：根据目前生物学及医学发展的需要，研制了一种在普通倒置生物显微镜下探测极微弱细胞荧光图象的高增益荧光显微镜。系统采用计算机采集荧光图象，由量化标定曲线对荧光图象中的每点根据其灰度值给出定量数据，解决了以往荧光显微镜只能定性观察不能定量分析的困难。A high-gain fluorescence microscope has been developed to meet the needs of medical and biological research. In this instrument,a computer is used to get fluorescence image of cells,and quantitative data at each point are obtained from its demarcating curve,and this is impossible for an ordinary fluorescence microscope.

关 键 词：荧光显微镜 荧光象增强器 CCD摄象机 量化处理 

分 类 号：TH742.65[机械工程—光学工程]