星状孢子素和金雀异黄素对人早幼粒白血病细胞系的诱导分化作用  

DIFFERENTIATION OF HUMAN PROMYELOCVTIC LEUKEMIA(HL-60) CELLS INDUCED BY STAUROSPORINE AND GENISTEIN

作　　者：董光宇[1] 战洪生[1] 颜卉君[1] 吴国利[1] 

机构地区：[1]北京师范大学生物学系,北京医科大学天然药物及仿生药物国家重点实验室

出　　处：《北京师范大学学报（自然科学版）》1995年第3期396-399,共4页Journal of Beijing Normal University(Natural Science)

基　　金：国家自然科学基金;北京医科大学天然药物及仿生药物国家重点实验室资助

摘　　要：以人早幼粒白血病细胞ＨＬ－６０细胞为模型，研究了星状孢子素和金雀异黄素对该细胞的诱导分化作用，结果表明，ＨＬ－６０细胞经２种药物分别处理后，细胞增殖能力均呈下降趋势，细胞形态学变化明显，大部分细胞趋于成熟，硝基兰四氮唑（ＮＲＴ）还原能力增强，ＮＢＴ阳性细胞百分率均达８０％以上，说明此２种药物对ＨＬ－６０细胞有明显的诱导分化作用。ＨＬ－６０细胞酸性磷酸酶活力显著升高，则揭示该细胞沿粒细胞方向趋于分化。Differentiation of human promyelocytic leukemia(HL-60) cell induced by staurosporine and genistein were studied using HL-60 cells as model. Proliferation of HL-60 cells were inhibited significantly after treated by staurosporine(10 μg.L-1) and genistein(10 μmol. L-1) for 5 days; the treated cells turned to be matured morphologically and NBT reduction ability was abtained. The activity of acid phosphatase in the treated HL-60 cells was increased significantly. The results showed that these drugs can induce differentiation of HL-60 cells along granulocyte lineage in vitro .

关 键 词：分化 星状孢子素 金雀异黄素 白血病 HL-60细胞 

分 类 号：R979.1[医药卫生—药品]