小纸层析法快速测定^（99m）Tc—HMPAO放射化学纯度  

Rapid determination of the radiochemical purity of ￣(99m)Tc-HMPAO by mini-paper chromatography

作　　者：薛方平[1] 顾兆祥[1] 林祥通[1] 

机构地区：[1]上海医科大学附属华山医院

出　　处：《核技术》1995年第11期690-692,共3页Nuclear Techniques

摘　　要：采用小纸层析系统和１：１的氯仿／四氢呋喃展开剂分析９９ｍＴｃ－ＨＭＰＡＯ的放射化学纯度，并与两个薄层层析系统和一个纸层析系统的方法作比较，配对ｔ检验结果表明两种方法测定的放射化学纯度无显著差异（Ｐ＞０．０５）；放射化学纯度在７４．２％－９６．４％（ｎ＝３０），小纸层析法与薄层／纸层析法紧密相关（ｒ＝０．９７，Ｐ＜０．００１），回归公式ｙ＝１．００６ｘ－０．３２３．A mini-paper chromatography (MPC) system has been developed to analyze the radiochemical purity of 99mTc-HMPAO involving a 1:1 chloroform/tetrahydrofuran developing solvent. The MPC procedure is compared with the method of two thin layer chromatographic system and one paper chromatographic system (TLC/PC). The results of the paired t-tests show that radiochemical purities measured by MPC and TLC/PC do not difference significantly (P>0.05). For radiochemical purity values ranging from 74.2%- 96.4% (n=30), the MPC and TLC/PC methods correlated closely (r= 0.97) with a regression equation of MPC (%) = 1.006 TLC/PC-0.323.

关 键 词：放射化学纯度 小纸层析法 脑血流灌注 显像剂 

分 类 号：R816.1[医药卫生—放射医学]