741杨离体叶片再生及抗虫基因转化  

Plant Regeneration of Excised Leaf from 741 Poplar and Transformation with Insect-Resistant B.t.toxin Gene

作　　者：郑均宝[1] 张玉满[1] 杨文芝[1] 裴东[1] 田颖川[1] 莽克强[1] 

机构地区：[1]河北林学院基础部,中国林业科学研究院林研所,中国科学院微生物研究所

出　　处：《河北农业大学学报》1995年第3期20-25,共6页Journal of Hebei Agricultural University

基　　金：中国科学院北京植物生物技术开放实验室及河北省教委资助

摘　　要：用７４１杨［Ｐｏｐｕｌｕｓａｌｂａ（ｐ．ｄａｖｉｄｉａｎａ×Ｐ．ｓｉｍｏｎｉｉ）×Ｐ．ｔｏｍｅｎｔｏｓａ）］试管植株无菌叶片作外植体，经研究，筛选了诱导不定芽分化、增殖和生根培养基；暗培养５～１５ｄ能提高不定芽分化的诱导率。无根或生根试管植株两类叶片均可用作外植体。建立了７４１杨最佳转化和再生系统。用含有完全改造的Ｂｔ基因表达载体ｐＢ４８．７转化优良杨树新品种－７４１杨，经在含有卡那霉素培养基上选择和ＰＣＲ检测已获得初步确定的转基因植株。Using leaves from aseptuly plantets of 741 poplar [Populus alba×(P.davidiana×P.simonii)× P.tomentosa] as the explants, the optimiu conditions for the induction of adventitious bud differentiation, multiplication and rooting were selected in this study. Dark culture for 5- 15 days could increase the rate of adventitious bus differentiation. meanwhile,the status of adventitious bud in rooted and rootless plantlets in vitro were compared. Finally, a best system for iran formation and regeneration of 741. poplar plants was established. The modified B. t. eaxin gene carried on PB 48. 7 was used to transform elite variety poplar 741.According to the selection in kanamycin contained media and PCR test,the plantlets with the transformed gene were created.

关 键 词：叶片外植体 再生 BT基因 转化 杨树 试管苗 

分 类 号：S792.110.4[农业科学—林木遗传育种]