人胎黑质细胞的冷冻保存  

Cyropreservation of substantia nigral cells of human fetus

作　　者：张华[1] 周明付[1] 林玲[1] 涂心明[1] 汤善钧[1] 聂正明[1] 周祥庭 

机构地区：[1]河南医科大学解剖学教研室,洛阳医学专科学校解剖学教研室

出　　处：《河南医科大学学报》1995年第3期231-233,共3页Journal of Henan Medical University

基　　金：河南省自然科学基金

摘　　要：采用台盼蓝染色法，观察了第３～６月人胎黑质细胞冷冻保存的细胞存活率，结果：①胎龄３～６月人胎黑质细胞冷冻保存后均可存活，但以胎龄３和４月细胞存活率较高。②液氮保存的细胞存活率不受保存时间的影响，而４℃保存的细胞存活率随保存时间的延长而逐渐降低。结果提示，液氮保存人胎黑质细胞的保存条件以胎龄３和４月为宜。The survival rate of substantia nigral cells in the human fetus of 3 to 6 months after cyropreserving was observed with trypan blue staining method. The results were as follows:①The cells of substantia nigra of the human fetus of 3 to 6 months might survive after cyropreserving. However,the survival rate of the fetus of 3 and 4 months was higher than that of 5 and 6 months.②The survival rate of the cells that were stored in liquid nitrogen was not affected by storing time, but the survival rate of the cells that were stored in 4℃ decreased gradually with the lengthening of the storing time.The results suggested that the optimal age for cyropreservation of substantia nigral cells of the human fetus stored in liquid nitrogen might be 3 and 4 months.

关 键 词：冷冻保存 人胎 胚胎组织移植 黑质细胞 

分 类 号：R321.5[医药卫生—人体解剖和组织胚胎学] R622.3[医药卫生—基础医学]