凝胶电泳DNA相对分子质量测定的软件设计  

Design a Software for Determining DNA Relative Molecular Mass by Gel Electrophoresis

作　　者：王绩[1] 傅丽霞[1] 

机构地区：[1]华中农业大学生命科学技术学院

出　　处：《湖北医科大学学报》1995年第4期395-397,共3页

摘　　要：本程序用于测定凝胶电泳ＤＮＡ片段的相对分子质量，利用数字化仪读出电泳照片中ＤＮＡ片段的位置，根据Ｓｏｕｔｈｅｒｎ的计算方法得出标准曲线方程．输入照片上ＤＮＡ片段的位置坐标，能立即得出其分子量，同时在屏幕上对应地显示出该ＤＮＡ片段．如有错误输入，能进行识别并立即修正，所有的分析结果自动形成一个表格显示在屏幕上，各ＤＮＡ片段的显示位置与电泳照片上的位置相对应．计算机显示的电泳图谱及结果分析报告，可由打印机输出或存入磁盘，系统由ＩＢＭ兼容机，打印机及数字化仪组成．This program is used to determine the relative molecular mass of DNA fragment by gel electrophoresis.The positions of the bands are read from gel photographs by means of a digitizer.Standard curves are calculated by the method of southern.The bands,as they are measured,are reproduced on the screen so that erroneous input can be recognized and corrected immediately.Similarly the estimated fragment sizes are printed in a table in the same relative positions as the bands on the gel.This make it especially easy to relate fragment sizes with the bands on the gel picture,The graphic and analysis report of the gel can be print out and save in disk.The system connected with PC comptiters,printer and digitiger.

关 键 词：凝胶电泳 DNA 相对分子质量 计算机 软件 

分 类 号：Q523[生物学—生物化学] TP311[自动化与计算机技术—计算机软件与理论]