小鼠卵母细胞孤雌激活及胚胎电融合研究  

Study On parthenogenology of oocytes and electrofusion of two-cell embryos in mouse

作　　者：赵浩斌[1] 陈东宝[1] 魏庆信[1] 

机构地区：[1]湖北省农科院畜牧兽医研究所

出　　处：《湖北农业科学》1995年第5期51-54,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金;湖北省自然科学基金

摘　　要：对小鼠的研究结果表明，７％酒精对１７ｈ和１９ｈ卵龄卵的激活率差异不显著（Ｐ＞０．０５），但１９ｈ卵激活后的卵裂率显著高于１７ｈ卵（Ｐ＜０．０１），酒精处理前去掉卵丘能使激活率提高到９０％～１００％，酒精处理时间（５ｍｉｎ和１０ｍｉｎ）之间差异不显著（Ｐ＞０．０５）。脉冲持续时间为４０μｓ，在连续３个电脉冲条件下，２００ｖ／ｍｍ场强的卵裂率最高，达７２．２％。２－细胞胚融合率在２００ｖ／ｍｍ前随电压升高而升高，２００ｖ／ｍｍ时达１００％。因此，在本试验条件下，小鼠卵母细胞激活及２－细胞胚电融合的最佳条件为２００ｖ／ｍｍ。Oocyte activation by alcohol or electric pulse and electrofusion of 2-cell em-bryos were studied in mouse. The results showed that①there was not difference in activa-tion effeciency between 17h and 19h of oocyte age by 7%alcohol,but the cleavage percentageof oocytes at 19h was clearly higher than oocytes at 17h after activation,activation percentagecan be raised to 90%～100%by removing cumulus cells before treatment with alcohol,thedifference was not clear between time of treatment with alcohol(5 min amd 10 min);②thehighest cleavage percentage(72.2%)was gotten at field strength 200v/mm,pulse duration4μs,3 continuous pulses;③the electrofusion percentage of 2.cell embryos was raised withfield strength before 200v/mm,100%fusion percentage was gained at 200v/mm. So that,theoptimal pulse voltage is 200v/mm for oocyte electroactivation and 2-cell embryo electrofusionof mouse under our experiment conditions.

关 键 词：小鼠 孤雌激活 胚胎 电融合 繁殖 

分 类 号：S865.133[农业科学—野生动物驯养]