组织培养在苎麻多倍体研究中的应用初报  

THE APPLICATION OF TISSUE CULTURE TO THE STUDY OF RAMIE POLYPLOID

作　　者：郑思乡[1] 罗慧敏[1] 董延瑜[1] 洪亚辉[1] 王春桃[1] 李宗道[1] 程尧楚[1] 刘国民[1] 

机构地区：[1]湖南农业大学苎麻研究所,湖南农业大学生物技术系,湖南农业大学农学系

出　　处：《湖南农业大学学报（自然科学版）》1995年第4期361-365,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金

摘　　要：研究了组织培养结合秋水仙素不同浓度和处理时间对苎麻试管苗的诱导效果，发现组织培养可以明显提高处理株的成活率，０．１％秋水仙素处理６ｄ仍有３０％左右的植株成活．首次采用不定芽技术稳定苎麻多倍体，获得了四倍体细胞达９４％的单株，并发现了三倍体和非归整倍体细胞．同时对处理过的试管苗进行了气孔鉴定和染色体检查，发现试管苗的气孔鉴定可靠性大．Tissue culture connected with traditional methods is used to studying ramiepolyploid. The inducing effects of colchicine of different concentration and treating time on tubeseedlings are compared. It is found that tissue culture can increase the survial rate of treated plantsthat 1/3 plants survives after 6 day-treatment with 1/1000 colchicine. It is the first time thatadventitious buds would be used to stabilize ramie polyploid ; and tetraploid plants with almosttetraploid cells, some triploid cells, aneuploid cells are ebtained. The tests of stomate dimensionsand chromosome numbers of treated plants are carried out, which shows that there are remarkabledifference among different ploidy plants in stomate dimension, which can be used as a reliableindex in identifring the ploidy of tube plants.

关 键 词：苎麻 多倍体 组织培养 

分 类 号：S563.103.5[农业科学—作物学]