糖的测定中值得注意的负值  

The Negative Value Noticed in Determination of Carbohydrates

作　　者：宋长铣[1] 

机构地区：[1]中国科学院江苏省植物研究所

出　　处：《湖南师范大学自然科学学报》1995年第3期55-58,共4页Journal of Natural Science of Hunan Normal University

摘　　要：研究发现，用常规系统分析方法测定植物材料中各类糖的含量时，非还原糖和多糖都有出现负值的情况。究其原因，是还原糖（葡萄糖和果糖）在水解的过程中发生变化：经２°／ＵＣ１１００℃水解３ｈ，葡萄糖损失１６．９６％，果糖损失６２．５１％，用芸苔花粉水提取液进行动力学试验，结果表明，在水解头１ｈ内，含糖量急遽降低，以后趋于平稳。The researches revealed that systimaticly determing various carbohydrates in plant material by routine method. the content of non-reducing sugars and polysaccharoses is negative value. It is reason to the reducing sugars(glucose and fructose)are changing in the.hydrolysis: the glucose loses 16. 96% and the fructose loses 62. 51% when they are.hydrolysed by 2% HCI for 3h at 100℃. The results of dynamatics test of water extract in Bird Rape (Brassica campestris 1)indicated that the carbohydrates content is lowered rapidly for the first lh in hydrolysis, and consequently, is stable at following time.

关 键 词：还原糖 负值 糖 测定 多糖 植物 

分 类 号：Q946.3[生物学—植物学]