牛精子F小体的检测  

DETECTION OF F-BODY IN SPERMATOZOA OF BULL

作　　者：张建湘[1] 高建华[1] 杜延龄 许德明[1] 

机构地区：[1]湖南医科大学组织学与胚胎学教研室,湖南省畜牧研究所,湖南省委行政处奶牛场

出　　处：《湖南医科大学学报》1995年第2期176-178,共3页Bulletin of Hunan Medical University

基　　金：国家自然科学基金

摘　　要：采用改良的蛋白酶消化方法，选择新鲜或冷冻牛精液，经Ｑ－Ｍ染色法检测牛精子Ｆ小体。结果发现：酶消化处理后随着染色时间延长（３０ｍｉｎ，６０ｍｉｎ，１２０ｍｉｎ），Ｆ小体检测率递增（２３．３±ｌ．３，４３．２±１．６，４６．６±１．０）；而未经蛋白酶处理者，Ｆ小体的检测率很低。资料表明，在牛部分精子头部能检测到Ｆ小体，可能与酶对精子膜的消化有关。消化后质膜对染料的通透性增高，高度螺旋状态的ＤＮＡ分子解聚，Ｙ染色体上的显色基团得以暴露。n attenlpt was made to identify the fluorescent body (F-body )with qouinacrine mus-lard(Q-M) staining in the spermatozoa of bull. The fresh and frozen semen were stainedwith Q-M for l20min; the rates of F-body were only 2.2 ±1. 4 and 3. 4± l.6 respective-ly. Both kinds of specimens treated with lX protease and then stained for 30, 60, 120min,therates of F-body were 23.3±1.3,43. 2±1.6 and 46.6±1. 0 respectively, These results led tothe conclusion that the F-body is present in Y sperm of bull. The successful detection of Fbody in the sperm of bull is attributed to:(l) prolonged time of specimen in the Q-M solu-tion for staining,(2) the treatment to the membrane and depolymerization of aItitude spiralDNA by protease digestion which yielded more favorable condition for staining in the speci-mens.

关 键 词：精子 性染色体 F小体 牛 

分 类 号：S814.1[农业科学—畜牧学]